Synaptic Systems - Endobrevin

Cat. No.: 104 303

Amount: 50 µg

Price: $460.00

Cat. No. 104 303	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: 1 : 100 up to 1 : 500 gallery IHC: yes IHC-P: not tested yet
Immunogen	Recombinant protein corresponding to AA 1 to 75 from rat Endobrevin (UniProt Id: Q9WUF4)
Reactivity	Reacts with: human (Q9BV40), rat (Q9WUF4), mouse (O70404). Other species not tested yet.
Specificity	K.O. validated PubMed: 25138214
Data sheet	104_303.pdf

The endothelial diapedesis synapse regulates transcellular migration of human T lymphocytes in a CX3CL1- and SNAP23-dependent manner.
Schoppmeyer R, van Steen ACI, Kempers L, Timmerman AL, Nolte MA, Hombrink P, van Buul JD
Cell reports (2022) 383: 110243. 104 303 WB; tested species: human

Plasma membrane flipping of Syntaxin-2 regulates its inhibitory action on insulin granule exocytosis.
Kang F, Xie L, Qin T, Miao Y, Kang Y, Takahashi T, Liang T, Xie H, Gaisano HY
Nature communications (2022) 131: 6512. 104 303 IP, WB; tested species: human,mouse

Defective AP-3-dependent VAMP8 trafficking impairs Weibel-Palade body exocytosis in Hermansky-Pudlak Syndrome type 2 blood outgrowth endothelial cells.
Karampini E, Schillemans M, Hofman M, van Alphen F, de Boer M, Kuijpers TW, van den Biggelaar M, Voorberg J, Bierings R
Haematologica (2019) : . 104 303 WB, ICC; KD verified; tested species: human

Syntaxin 8 regulates platelet dense granule secretion, aggregation, and thrombus stability.
Golebiewska EM, Harper MT, Williams CM, Savage JS, Goggs R, Fischer von Mollard G, Poole AW
The Journal of biological chemistry (2015) 2903: 1536-45. 104 303 WB

Vesicle associated membrane protein 8 (VAMP8)-mediated zymogen granule exocytosis is dependent on endosomal trafficking via the constitutive-like secretory pathway.
Messenger SW, Falkowski MA, Thomas DD, Jones EK, Hong W, Gaisano HY, Giasano HY, Boulis NM, Groblewski GE
The Journal of biological chemistry (2014) 28940: 28040-53. 104 303 WB, IHC; KO verified

Cat. No.: 104 303

Amount: 50 µg

Price: $460.00

ICC

Background

Protocols

Endobrevin (Vamp8) antibody - 104 303 K.O.